[circDDX17 targets miR-223-3p / RIP3 to regulate the proliferation and apoptosis of non-small cell lung cancer cells].

Objective: To explore the molecular mechanism of circDDX17 regulating the proliferation and apoptosis of non-small cell lung cancer cells by targeting the miR-223-3p/RIP3 molecular axis. Methods: The expression levels of circDDX17, miR-223-3p, and RIP3 in human normal lung epithelial cell lines BEAS-2B and non-small cell lung cancer cells H1299, A549, and H446 were detected by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The plasmids of pcDNA, pcDNA-circDDX17, anti-miR-con, anti-miR-223-3p, pcDNA-circDDX17 and miR-con, pcDNA-circDDX17 and miR-223-3p mimics were transfected into H1299 cells. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay was used to detect the cell proliferation. Flow cytometry was used to detect the cell cycle and cell apoptosis. Plate cloning experiment was used to detect cell proliferation ability. The dual luciferase report experiment was applied to verify the targeting relationship between miR-223-3p with circDDX17 and RIP3. Western blot was used to detect the protein expression of cyclinD1, CDK2, cleaved caspase-3 and Bax. Results: The expression levels of circDDX17 and RIP3 mRNA in H1299, A549, and H446 cells were significantly reduced (P<0.05), the expression level of miR-223-3p mRNA was significantly increased (P<0.05) compared with BEAS-2B. The cell viability [(69.46±4.68)%], the number of cell clones (83.49±7.86), the proportion of cells in S phase [(22.52±1.41) %], the protein expression levels of cyclinD1 and CDK2 in PCDNa-CircDDX17 group were lower than those in pcDNA group [(97.54±7.72)%, 205.03±13.37, (28.69±1.49)%, respectively, P<0.05], while the percentage of G0/G1 phase cells [(64.45±3.56)%], apoptosis rate [(18.36±1.63)%], the protein expression levels of cleaved caspase-3 and Bax in pcDNA-circDDX17 group were higher than those of pcDNA group [(51.33±2.76) % and (5.21±0.54) %, respectively, P<0.05]. The viability [(72.64±5.44)%], the number of cell clones (78.16±8.23), the proportion of S-stage cells [(21.34±1.59) %], the protein expression levels of CyclinD1 and CDK2 in anti-miR-223-3p group were lower than those in anti-miR-con group [(103.47±6.25)%, 169.32±14.53, (28.43±1.26)%, respectively, P<0.05]. Percentage of G0/G1 phase cells [(62.86±3.28)%], apoptosis rate [(14.64±1.67)%], the protein expression levels of cleaved caspase-3 and Bax in the anti-miR-223-3p group were higher than those of anti-miR-con group [(51.33±2.71)% and (4.83±0.39)%, respectively, P<0.05]. MiR-223-3p has complementary sites with circDDX17 or RIP3. The viability [(135.45±9.28)%], the number of cell clones (174.64±10.68), the proportion of S-phase cells [(26.39±2.25)%], the protein expression levels of cyclinD1 and CDK2 in pcDNA-circDDX17+miR-223-3p group were higher than those in pcDNA-circDDX17+miR-con group [(101.56±6.68)%, 107.65±7.62, (21.64±1.72)%, P<0.05]. Percentage of G0/G1 phase cells [(56.64±2.76)%], apoptosis rate [(8.34±0.76)%], the protein expression levels of cleaved caspase-3 and Bax in pcDNA-circDDX17+miR-223-3p group were lower than those of pcDNA-circDDX17+miR-con group [(64.03±3.48)% and (15.21±1.18)%, respectively, P<0.05]. Conclusion: circDDX17 could inhibit the proliferation and induce apoptosis of non-small cell lung cancer cells via targeting the miR-223-3p / RIP3 molecular axis.

Effects of powder size and preset thickness on the microstructure and properties of Ni35 thin coating prepared by laser cladding.

Ni35 thin coatings were prepared on 40Cr steel by preset powder laser cladding technology. The effects of powder size and preset thickness on the dilution rate, microstructure, and properties of the coating were systematically studied. The results showed that the coating with smaller powder size and larger preset thickness had smaller grain size, denser microstructure, a flatter bottom of the molten pool, and lower dilution rate. The micro-hardness of the coating with large powder size and preset thickness was higher. The friction coefficient of the coating with small powder size and small preset thickness was smaller, but the latter had larger curve fluctuation and wear volume loss. The corrosion rate of the coating was high during the first 24 h, then relatively stable after 68 h, and the corrosion rate and loss of the coating with small powder size and large preset thickness were smaller.

MiR-4282 inhibits proliferation, invasion and metastasis of human breast cancer by targeting Myc.

OBJECTIVE: To investigate the expression and role of MicroRNA 4282 (miR-4282) in the development of breast cancer. MATERIALS AND METHODS: In situ hybridization was performed to investigate the expression of miR-4282 in human breast cancer tissues. Cell lines stably over-expressed miR-4282 were established by lentivirus transfection. The effects of miR-4282 on the biological behavior of breast cancer cells were then explored in vitro. RESULTS: The results showed that miR-4282 was down-regulated in human breast cancer tissues and was particularly in invasive and metastatic tumors. In addition, the expression of miR-4282 was related to the occurrence of metastasis and the clinical grade of breast cancer. Recovery of miR-4282 expression in the cell could inhibit the proliferation of breast cancer cells, blocked G1-S phase and promoted breast cancer cell apoptosis, inhibited breast cancer cell migration and invasion. Besides, miR-4282 also significantly enhanced the sensitivity of breast cancer cells to paclitaxel. The results of bioinformatics analysis combined with qRT-PCR and Western blot demonstrated that Myc might be the target gene of miR-4282 in breast cancer. CONCLUSIONS: miR-4282 inhibited the occurrence and development of breast cancer by regulating Myc, which made it a new target for clinical diagnosis and treatment of breast cancer.

[Epidemiological features of heatstroke in Shaoxing, China in 2008-2014].

OBJECTIVE: To investigate the epidemiological features of heatstroke in Shaoxing, China in 2008-2014, and to provide a reference for developing prevention and control measures for heatstroke. METHODS: The reports on heatstroke in Shaoxing in 2008-2014 were collected from the China Information System for Disease Control and Prevention, and the data were summarized and analyzed. RESULTS: A total of 1 676 heatstroke cases were reported in Shaoxing in 2008-2014, among which 1 485 cases(88.6%) were mild heatstroke and 191 cases(11.4%) were severe heatstroke. Nine persons died of severe heatstroke, and the fatality rate was 0.5%(9/1 676). Among these persons, 4(44.4%, 4/9) were the elderly living at home, 4 (44.4%, 4/9) were farmers (working outdoors), and 1(11.2%, 1/9) was unemployed. The male patients with heatstroke accounted for 66.3%(1 111/1 676), and those with severe heatstroke accounted for 13.1%(146/1 111); the female patients with heatstroke accounted for 33.7%(565/1 676), and those with severe heatstroke accounted for 8.0%(45/565); there were significant differences between male and female patients(χ(2)=9.94, P=0.002). The mean age of patients with heatstroke was 48.7±18.8 years; the mean age of male patients was 49.8±17.9 years, and that of female patients was 46.6±20.3 years, with a significant difference between the male and female patients(t=3.19, P<0.001). Most of the patients experiencing heatstroke were in their forties or fifties, each accounting for 19.2%(321/1 676); in male patients who experienced heatstroke, those in their fifties accounted for the highest percentage(21.5%, 239/1 111), and in female patients who experienced heatstroke, those in their twenties accounted for the highest percentage (18.9%, 107/565). Based on stratification by age, the severity of heatstroke differed significantly between male and female patients in their forties, fifties, sixties, seventies, and eighties(P<0.05). Most of the cases of heatstroke occurred in June to September, accounting for 98.9%(1 657/1 676), particularly in July and August, accounting for 87.1%(1 460/1 676). CONCLUSION: In Shaoxing, heatstroke usually occurs in summer, and most cases are mild. Middle-aged and elderly men and farmers(working outdoors) are susceptible to heatstroke, and warnings on heatstroke and emergency treatment of severe heatstroke should be enhanced.

Visualization and body distribution of [¹³¹I]-herceptin in nude mice with BT-474 breast carcinoma.

The study aimed to investigate the bio-distribution and radio-immuno-imaging features of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. [(131)I]-Herceptin was administrated by tail intravenous injection to the nude mice with BT-474 breast carcinoma. Radiocounting was performed at 4, 12, 24, 48, and 96 h after administration. The activity ratio in the tumor tissue and non-tumor tissue (T/NT) and the radiocounting percentage per gram tissue to the injected dose (%ID/g) were calculated. The nude mice with BT-474 breast carcinoma were also visualized continuously by single photon emission computed tomography at 2, 4, 8, 12, 24, 48, and 96 h after the injection of [(131)I]-herceptin. Nude mice with MDA-MB-231 used as the control group were subjected to the same analyses. Clear tumor images were obtained after the injection of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. The images were the clearest at 24 h after the injection and remained clear even at 96 h. The T/NT ratio and %ID/g in the tumor tissues of nude mice with BT-474 were both significantly higher than those of the control group (P < 0.01). [(131)I]-Herceptin displays tumors clearly in the nude mice with BT-474 and accumulates well in the tumor tissues.

Starting hemoglobin value predicts early phase prognosis after liver transplantation.

Few studies have addressed the relationship between starting hemoglobin (Hb) value and early phase prognosis after liver transplantation (OLT). The aim of our study was to determine the relationship between the starting Hb value and the early phase prognosis after OLT by retrospectively reviewing the medical records of 102 consecutive recipients. Within this cohort, 47 patients had pulmonary complications after OLT, including eight cases of pulmonary edema, 12 cases of acute lung injury, six cases of acute respiratory distress syndrome, and 21 cases of pneumonia. According to whether the patients had pulmonary complications or not, they were categorized into the "no" versus the pulmonary complication groups. Twenty-two perioperative variables were analyzed in both groups to screen for variables that affected early pulmonary complications. A starting Hb ≤ 100 g/L was an independent risk factor for postoperative pulmonary complications. The duration to initial passage of flatus and the intensive care unit length of stay were significantly prolonged in patients with starting Hb values ≤ 100 g/L; these patients had poorer arterial blood gas analyses. The starting Hb value predicted the early phase prognosis after OLT for cirrhosis-associated hepatocellular carcinoma.

Treatment of acute bronchiolitis with Chinese herbs.

In a randomised single blind trial the Chinese herbs Shuang Huang Lian were evaluated for the treatment of acute bronchiolitis. Children with acute bronchiolitis and serological evidence of recent respiratory syncytial virus infection were studied in a tertiary hospital in Harbin, China. The 96 children were randomised into three treatment groups: herbs, herbs with antibiotics, and antibiotics alone. The herbs were prepared by the medical school pharmacy and administered daily by intravenous infusion for seven days. The main outcomes, assessed blindly, were symptomatic improvement in cough, fever, wheezing, chest signs, and duration of stay in hospital. The mean duration of symptoms from the start of treatment was 6.2 (confidence interval 5.6 to 6.9) days in the two groups treated with herbs compared with 8.6 (confidence interval 7.5 to 9.8) days in the group treated with antibiotics alone. The mean reductions in duration of clinical manifestations for treatment with antibiotics alone compared with herbs were: from 3.1 to 1.5 days for fever, 9.1 to 6.1 days for cough, 6.5 to 4.1 days for wheezing, and 7.2 to 4.9 days for chest crackles. No adverse effect of Shuang Huang Lian herbal treatment was detected. In conclusion, this study confirms Chinese experience with Shuang Huang Lian that it is safe and effective, and warrants further study.